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Proteinase K

lyophilised, ≥30 mAnson U/mg
Isolated from fungi (Tritirachium album).
Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.
Storage temp.: +4 °C
WGK 1

A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.

Suitable for:
Preparing chromosomal DNA for PFGE
Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C.
Over +65 °C, inactivation due to denaturation.
Stability: pH 4.0-12.5.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Activators: Denaturating agents (SDS, urea).
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol.
Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.

Application:
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Working solution: 50 µg/ml
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B1017-1g
Availability
509.45€
B1017-5g
Availability
2021.75€
B1017-100mg
Availability
90.20€
B1017-250mg
Availability
169.00€
B1017-500mg
Availability
301.40€
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Description

DNAs free

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